Figure 8From: Porcine congenital splayleg is characterised by muscle fibre atrophy associated with relative rise in MAFbx and fall in P311expressionEffects of P311 on C2C12 cell proliferation and differentiation. (A) BrdU assay was performed on P311-infected and adenovirus-GFP control cells, as well as P311-transfected and vector only-transfected cells. For both approaches, P311 over-expression significantly increased cell proliferation. (B)P311-stably transfected C2C12 myotubes (differentiated for 9 days) were separately immunostained for desmin and MYH fast, along with DAPI nuclei staining. Under immunofluorescence, myotubes appeared less abundant in P311-transfected cells. (C) Fusion index quantification on immunostained images showed that P311-transfected cells resulted in less mytoube formation than control cells. Asterisks indicate statistical significance. BrdU assay and fusion index determination were analysed with SAS software, differences between pair-wise combinations of the least square means were tested for significance (*p < 0.05). Error bars were standard error, n = 3 replicates from 3 independent experiments.Back to article page