Primer | Sequence (5′–3′) | Aim |
---|---|---|
MB-F | GCTTCATGTGGTGATAAATACTTTA | Identify P48 gene |
MB-R | CTATTTTTGTGTTTCTTTAGCCAAT | |
MB-a | CATGCCATGGGCGCTTCATGTGGTGATAAATACTTTA | Mutate point 192 |
MB-a’ | TGAAATTTTATGAACTGCTTCCCAACCAGATTGATTG | |
MB-b | TCAATCAATCTGGTTGGGAAGCAGTTCATAAAATTTC | Mutate point 648 |
MB-b’ | CAGAAACTGCTGGCCAAGGAATACCACCGAAGG | |
MB-c | CCTTCGGTGGTATTCCTTGGCCAGCAGTTTCTG | Mutate point 1275 |
MB-c’ | CAGCAGGTTGGTCTGGCCATTTTGTACCTAAAGC | |
MB-d | CTTTAGGTACAAAATGGCCAGACCAACCTGCTGACC | Mutate point 1317 |
MB-d’ | CTATTTTTGTGTTTCTTTAGCCAACCAGTTAATCATTTTC | |
MB-e’ | GCTTCTGGGTGTTCTTTATTCCAGTCAATAATACCTTGG | Mutate point 699 |
MB-f | CATTCCAAGGTATTATTGACTGGAATAAAGAACACCC | Â |