Figure 5
Panel A – PCR primers designed against rat SUR1 amplified a single band of the expected size (248 bp) from each of the three canine insulinomas examined (lanes labeled SUR1 1–3). Sequence analysis confirmed the identity of each band as SUR 1. GAPDH expression was used as a psoitive control (lanes labeled GAPDH 1–3). Semiquantitative analysis showed that SUR1 expression by insulinoma #3 was 3.5 and 2 fold greater than insulinomas #1 and #2, respectively (see text for explanation). Size markers (M) are shown on the left. The arrows indicate the approximate location of the 300 bp marker. Panel B – PCR primers designed against canine Kir6.2 amplified a single band of expected size (371 bp) from normal pancreas (P) and from each of the three canine insulinomas (labeled 1–3) examined. Sequence analysis confirmed the identity of each band as Kir6.2. Size markers (M) are shown on the left. The arrows indicate the approximate location of the 400 bp marker